expression plasmid pmscv puro ires gfp 64 (Addgene inc)
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Addgene inc
expression plasmid pmscv puro ires gfp 64
Expression Plasmid Pmscv Puro Ires Gfp 64, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmscv+pig/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/10__1016_slash_j__isci__2026__115182-641-41-45
Average 93 stars, based on 77 article reviews
Expression Plasmid Pmscv Puro Ires Gfp 64, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmscv+pig/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/10__1016_slash_j__isci__2026__115182-641-41-45
Average 93 stars, based on 77 article reviews
expression plasmid pmscv puro ires gfp 64 - by Bioz Stars,
2026-09
93/100 stars
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Amplification:Article Title: Metabolic immunity to infection is driven by mitochondrial one-carbon metabolism Article Snippet: .. Human MTHFD2 cDNA amplified from ES-2 cells was cloned into Article Title: Mitochondria protect against an intracellular pathogen by restricting access to folate Article Snippet: .. Human MTHFD2 cDNA amplified from ES-2 cells was cloned into Clone Assay:Article Title: Metabolic immunity to infection is driven by mitochondrial one-carbon metabolism Article Snippet: .. Human MTHFD2 cDNA amplified from ES-2 cells was cloned into Article Title: A TP53 Intron-Derived Peptide Promotes Tumor Survival Article Snippet: For the generation of shRNA-mediated stable IDP knockdown, pTRC2 vector was first edited to have a KpnI site and then digested with KpnI and EcoRI. shRNA oligonucleotides were obtained from Intergrated DNA Technologies (IDT, USA), annealed, and ligated with vector using T4 ligase. .. After transformation, clones were screened through DNA extraction and Sanger Sequencing. shRNA oligonucleotides can be found in Table S1. Article Title: Outer membrane protein 25 of Brucella suppresses TLR-mediated expression of proinflammatory cytokines through degradation of TLRs and adaptor proteins. Article Snippet: The levels of proinflammatory cytokines in the culture supernatants were quantified using Quantikine Duo ELISA kit (R&D Systems) as per the manufacturer’s protocol. .. To generate lentivirus particles harboring Omp25d expression plasmid, Omp25d was cloned into the lentivirus expression plasmid, Article Title: Mitochondria protect against an intracellular pathogen by restricting access to folate Article Snippet: .. Human MTHFD2 cDNA amplified from ES-2 cells was cloned into Generated:Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. Generating TCR hybridomas We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Retroviral:Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. Generating TCR hybridomas We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Plasmid Preparation:Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. Generating TCR hybridomas We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Article Title: Outer membrane protein 25 of Brucella suppresses TLR-mediated expression of proinflammatory cytokines through degradation of TLRs and adaptor proteins. Article Snippet: The levels of proinflammatory cytokines in the culture supernatants were quantified using Quantikine Duo ELISA kit (R&D Systems) as per the manufacturer’s protocol. .. To generate lentivirus particles harboring Omp25d expression plasmid, Omp25d was cloned into the lentivirus expression plasmid, Transduction:Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. Generating TCR hybridomas We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Construct:Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. Generating TCR hybridomas We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Purification:Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. Generating TCR hybridomas We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Article Title: Mapping the T cell repertoire to a complex gut bacterial community Article Snippet: .. We generated the retroviral vector pMSCV-mCD4-PIG TCR-OTII to transduce the NFAT-GFP 58α − β − hybridoma cells with synthetic TCR constructs. Transformation Assay:Article Title: A TP53 Intron-Derived Peptide Promotes Tumor Survival Article Snippet: For the generation of shRNA-mediated stable IDP knockdown, pTRC2 vector was first edited to have a KpnI site and then digested with KpnI and EcoRI. shRNA oligonucleotides were obtained from Intergrated DNA Technologies (IDT, USA), annealed, and ligated with vector using T4 ligase. .. After transformation, clones were screened through DNA extraction and Sanger Sequencing. shRNA oligonucleotides can be found in Table S1. DNA Extraction:Article Title: A TP53 Intron-Derived Peptide Promotes Tumor Survival Article Snippet: For the generation of shRNA-mediated stable IDP knockdown, pTRC2 vector was first edited to have a KpnI site and then digested with KpnI and EcoRI. shRNA oligonucleotides were obtained from Intergrated DNA Technologies (IDT, USA), annealed, and ligated with vector using T4 ligase. .. After transformation, clones were screened through DNA extraction and Sanger Sequencing. shRNA oligonucleotides can be found in Table S1. Sequencing:Article Title: A TP53 Intron-Derived Peptide Promotes Tumor Survival Article Snippet: For the generation of shRNA-mediated stable IDP knockdown, pTRC2 vector was first edited to have a KpnI site and then digested with KpnI and EcoRI. shRNA oligonucleotides were obtained from Intergrated DNA Technologies (IDT, USA), annealed, and ligated with vector using T4 ligase. .. After transformation, clones were screened through DNA extraction and Sanger Sequencing. shRNA oligonucleotides can be found in Table S1. shRNA:Article Title: A TP53 Intron-Derived Peptide Promotes Tumor Survival Article Snippet: For the generation of shRNA-mediated stable IDP knockdown, pTRC2 vector was first edited to have a KpnI site and then digested with KpnI and EcoRI. shRNA oligonucleotides were obtained from Intergrated DNA Technologies (IDT, USA), annealed, and ligated with vector using T4 ligase. .. After transformation, clones were screened through DNA extraction and Sanger Sequencing. shRNA oligonucleotides can be found in Table S1. other:Article Title: BRAF D594A mutation defines a unique biological and immuno-modulatory subgroup associated with functional CD8 + T cell infiltration in colorectal cancer Article Snippet: THBS1-overexpressing plasmid (pMSCV-THBS1) was constructed Article Title: BRAF D594A mutation defines a unique biological and immuno-modulatory subgroup associated with functional CD8 + T cell infiltration in colorectal cancer. Article Snippet: THBS1-overexpressing plasmid (pMSCV-THBS1) was constructed Expressing:Article Title: Outer membrane protein 25 of Brucella suppresses TLR-mediated expression of proinflammatory cytokines through degradation of TLRs and adaptor proteins. Article Snippet: The levels of proinflammatory cytokines in the culture supernatants were quantified using Quantikine Duo ELISA kit (R&D Systems) as per the manufacturer’s protocol. .. To generate lentivirus particles harboring Omp25d expression plasmid, Omp25d was cloned into the lentivirus expression plasmid, |